Plasmids | Genetics | Biology

[JejeBabe]

very interesting loads of crap sir

[grisgrisy]

good work here

[Vstar94]

That's all very nice, but how can I shoot lightning from my hand?

[konvict501]

bioshock will be here soon

[insomniacgrace]

Thank you very much for sharing this video clip. It helped me a lot towards better understanding about these Plasmids.

[ckbabyyable]

THANK YOU! THANK YOU, THANK YOU!

[quentain1]

I want to be injected with plasmids specificaly ice, telekenesis, fire,insect wearm,cyclone trap,hypnotizr big daddy(so i can shoot goo at people),enrage,electro bolt OR fire (none of the others though)

[MrGameOwnage]

half an hour before my exam and this just single handed taught me plasmids. Brilliant

[Chiparatualin]

Now I need a mutation!Evolve today!

[AlistairAI]

"Learn, would you kindly?"

[radioactivesoup200]

Now if only I can shoot lighting/bees/ice/fire oh that would so cool just hope I won't go crazy

[swiftedg]

@radioactivesoup200 nah a bit of bees never hurt anyone... wait...

[ariabm44]

can somebody explain me  how we transform the eycariotic shells with the recombinant dna?

[NorthForkFisherman]

@ariabm44 If you are going to use a prokaryotic plasmid in a eukaryote you need to modify it to match the linear chromosomes. That is, you will need a similar origin of replication, centromeres, and teleomeres that will allow your new artifical chromosome to replicated in step with the host cell. There's a lot of other small steps, but that's really all there is to it in the broad strokes.

[7555sundar]

you got video's about restriction enzymes forming staggered cut ends

[AbidinAliev]

you got video's about restriction enzymes forming blunt ends, in stead of sticky ends?

[ariabm44]

@AbidinAliev what the blunt ends are?

[SkavLifeB]

@ariabm44

Blunt ends are just another term for DNA regions that are cut by restriction enzymes.

If you noticed at 1:42, after the plasmids were cut, there were partial pieces of ssDNA, where the preferred gene segment attached to, on the ends? Those are considered sticky, and can be binded easily by a Ligase. Blunt ends on the other hand are very much like the name implies. There are no hanging segments, so gene fragments must bind directly to the end piece, which can prove much more difficult.

[ariabm44]

@SkavLifeB Thank u very much!

[CaptainFalconOnAPogo]

How do I shotted electro bolt?

[lynnandpete]

HOLY CRAP... WE CAN HAS BE SPLICED NOW?

[george77772moons]

Wow, Bioshock shock was actually somewhat accurate! IT ALL MAKES SENSE NOW!

[maginathan]

like in bioshock?

[masterpoop678]

Will this make me shoot lightning/fire/bees or be able to use telekineses if I inject myself with them 0.o

[ventilize]

@masterpoop678 now that you mentioned it I TOO am wonderingabout your question

[neilrulz24]

Soooo useful. Thanks!

[mavin174]

Thanks so much !