extracting my own DNA at home

[juanrojas26]

AAAAHHHH!!!!!!  no shots

[st3v33100]

Thank you !!

[Lvent2101]

i love biology but i cant take it see blood damn !

[st3v33100]

@Lvent2101 ,Well, i think you should practice to see blood. Good luck!

[seunketchup88]

You could see the twisted *SUPER MARIO THEME* GODDAMNITT!! I lold.

[vOuijaa]

I don't have dna, I'm a robot

[sundaypiglet]

Does ispropanol work on RNA?

[brenton015]

LOl i love it when you phone rings god damit

[wawa1196]

could you do it from saliva instead of blood

[500550arnob]

Does adding oil in stead of that other stuff work?

[ClimateScam]

I've been extracting my own DNA at home since the age of 12, if ya know what I mean ;)

[TabbytabtabOmega]

@ClimateScam i read this coment and i loled so hard!

[2t22tornadosiren]

@ClimateScam If you can ejaculate at 12 years old, you have early puberty.

[fredxable]

sweet vid

[bobtheowl2]

does this yield enough to visual in some form on a gel, even if just a smear, without using PCR?

[swoop155]

haha I love the part where she's like extracting her blood and she was like "well I guess this isn't for the faint of heart" LOL maybe a bit too late now :D Awesome video !

[hamster130]

"as you can see the twisted -phone rings- "god dammit" lmao!! got me cracking up. Great video. 

[ppark20]

LMAO @ the super mario ringtone

[nathanmarto]

.. "god damn it" .. lol

[samljer]

Seen this same thing, but the guy used strawberry juice and extracted DNA from a berry. I guess u can use almost anything :D

[bionerd23]

@samljer

well, any living thing has DNA. :)

no matter if a plant / fruit, a puddle of bacteria, some yucky fungus growing on your bread, a pig, or a human. but meat / muscle cells are much harder to break up than plant cells, so you'd have a harder time extracting cow DNA from a steak than e.g. extracting DNA from the salad sidedish. :P

[kwilliams3161]

what happens to your vain after you take the blood sample?does it repair or permanently stay damaged?



[cortexedge]

@kwilliams3161 My guess it's it repairs itself

[cortexedge]

@kwilliams3161 My guess it's it repairs itself

[supercooled]

@kwilliams3161 From what I learned a long time ago, basically the vein is perforated and like a wound, white blood cells travel to the wound and sticks itself forming a scab although in this case, it's very small so you can't see it like you would a scab on your leg in more common terms. White blood cells then blocks red blood cells from escaping and likely the area just heals itself within a few days.

[QuinnMorgendorffer]

Hey I did the experiment with Methylated Spirits. I had a look on google and there was an experiment using a kiwi fruit and it said Methylated Spirits were ideal for this. I can't see why that'd change for kiwi fruit, so I assumed it'd work for this experiment as well.

Unfortunately I couldn't get the dna to appear. However there was one more thing that may be the issue.

I used 45mL of water and one tablespoon (assumed US - 15mL) of salt. However all the salt didn't dissolve, are these right?

[WRSvideos]

Just a thought, instead of using your own blood, you can buy a cheap brand of unprocessed and uncooked meat from a store and use the blood from that, I did that for my science project and I tell ya, they don't expect this stuff from a 12 year old!

[QuinnMorgendorffer]

Hey Bionerd23, another brilliant video. I was just wondering is there anything else you can use other than Isopropanol? I'm by no means a biologist but I figured you use it as it wouldn't harm the DNA and it is less dense than the salt solution - so would I be able to use methanol or ethanol to do the same job?

I tried looking for Isopropanol at the chemist but they only sold huge bottles which is why I ask.

[bionerd23]

@QuinnMorgendorffer

hmmm. i'm not a chemist, but i'd expect that ethanol and methanol could work as well... if google doesnt know, there's one way to find out: try it! :P

by the way, cant you get isopropanol at household stores, doesnt e.g. rubbing alcohol contain that?

[QuinnMorgendorffer]

@bionerd23

I've only looked in chemists but I'll go have a look in some other stores tomorrow. Rubbing alcohol is what I was looking at in the pharmacy but sometimes it is ethanol rather than isopropanol. But yeah they only came in $10 bottles - something which I can't really afford for a fun little experiment at the moment :(

[QuinnMorgendorffer]

@bionerd23

Forgot to say though, if I can't find it I'll look for some other alcohols and let you know!

[onexdata]

@bionerd23 It actually works fine with only soap, spit and vodka, (any clear alcohol around 50 proof (25% alcohol) or higher works perfect, any soap with sodium laural sulfate listed in the top 3 ingredients works). The soap breaks open your cell membranes, and the alcohol attracts the long DNA because it's water-phobic. Salt is optional for filtering (coffee filter will do nothing for spit unless you just ate), and eye contact cleaner works much better than salt. Steps to follow

[onexdata]

@onexdata 1) Rub your cheeks with your tongue for 20 seconds and spit collected saliva into a shot glass 4 times, the shot glass should be 25% full, if not, repeat.

2) Drop 4-5 drops of dish soap into the glass, use a toothpick to thoroughly mix the soap with your spit, breaking open all cells and spilling their DNA.

3) Tilt the shot glass and pour in 75% vodka, be careful NOT to mix the vodka, it should sit on top. DNA will push itself into the alcohol because it's water-phobic.

Boom, done.

[onexdata]

@onexdata To collect your DNA from the shot glass for viewing in a microscope, staining, or sorting out the chromosomes, etc., insert a toothpick (DNA will "stick" better to wood than plastic) at an angle and twist in the same direction until you spool your DNA from the cloudy-whispy strands into a very long, thick single strand of all your DNA. There is no easier or faster way to collect your DNA.

[onexdata]

@onexdata It is very important to use a thin glass, a test-tube works best, but a shot glass works great. This is because DNA is mildly water-phobic (it avoids water), and therefore is attracted to alcohol, the smaller the volume and surface area the DNA has to work in, the greater and faster the effect happens. If your glass is thin enough, and alcohol strong enough the DNA will rise to the very top. eye contact cleaner helps isolate DNA from organelles and lipid fragments (proteins).

[onexdata]

@onexdata The contact cleaner contains a protease, which breaks down proteins, which all the enzymes, organelles and other cell soup the dish soap spilled out of the cells mostly is. Meat tenderizer, acids like pineapple juice and other common things contain protease. This final protease addition is ONLY for isolating your DNA for more advanced steps like staining (some proteins will stain along with your DNA, or isolating chromosomes for even more advanced stuff).

[bionerd23]

@onexdata

wow, awesome! i redid this experiment recently, but used "proper" protease (lab ware). watch?v=GfE04tpaqxU

...i didnt know contact lens cleaner contains protease, though! thank you very much for that information - and the simplified experiment instructions itself!

[swadow54]

amazing now u will be able to make yer own clone troopers :o

[MadManMarkAu]

Super Mario Bros ringtone!

[09fod]

outch ! that looked like it hurt :/

[lolololzsadface]

can you drink it

[flitzpiepensation]

How did u realize to zoom on ur DNA like that with a optical microscope? :-O I wanna try ur experiment too

[marianile]

coooool

good job out there!

[00011theman]

You coukd try to get a amp from ebay  to put in the dna extracted then you repeat it over and over and you will be able to see it under a microscope ;) Yet I know this and im only 12...

[famousvim26]

As you said, red blood cells do not have a nucleus, and thus no dna. Otherwise we wouldn't be able to transfuse blood to each other...! =P

[nv1860]

ok got the DNA part,

is it possible to get chromosones extrcted and viewable?

[bionerd23]

@nv1860

certainly not using a light microscope, nope, sorry...

[nv1860]

@bionerd23

otay,

how about any homebrew methods?

thank you

[bionerd23]

@nv1860

not possible unless you can build an electron microscope at home.

[nv1860]

@bionerd23

lool, ok, just had to ask =)

[PsychoticBovine]

that watch is cool, what kind is it? I have a thing for timepieces.

[bionerd23]

it's a "0I - THE ONE" binary watch. :)

[PsychoticBovine]

@bionerd23 awesome!

[polonium9]

same.

[Crotranee]

Are you work like medic? nice where did you got all that medic instruments :S

[bionerd23]

i got that stuff from a medical mailorder and scientific supply store, no problem in my country...

[vitorix24]

@bionerd23 lol... you live in germany and I live in Portugal... IT's UE... XD

[princessmj]

I wonder how much blood you've lost from doing this experiment!!! lol jk

Thanks though! it's amazing!

[919664]

kannst du ein deutsches video machen ?

[bionerd23]

kann ich schon, aber dann verstehen es die meisten meiner subscriber ja nicht mehr...

[biochem56]

lol i love how u used yr blood

[KarbineKyle]

Very Nice! I Love the experiments you do! They are so fascinating! Nice microscopic pictures too! Incredible! 5/5!

[p990ias]

and you can see the twisted... god damnit

[sublime361]

Thanks for posting!

[MismeretMonk]

This is nice! I want to do it also!

[chao129]

teehee, inadequate waynes.

[EatzingZureSchvideos]

Da na na na na Na - God Dammit...

lol

[phrog1337]

It should be quite possible to continue on and show that the the structure of dna is helical. Shining laser light on it should produce a diffraction pattern Taking the inverse fourier transform of the diffraction pattern might reveal some of the structure of the dna, assuming that the diffraction pattern and the picture you took of it is of decent resolution. You could probably do this at home, and take the inverse of the pattern using basic software like ImageJ.

[bionerd23]

that's also a good idea - a laser as light source. would have to be careful where to point it ^_^, but yeah, i'll just use the PC ocular / camera to look through the scope, anyway.

[monkeytwineball]

I thought that the sodium unwound the structure? Wouldn't that make it no longer helical, or have I misunderstood something?

[malicios]

Histones are what packages the dna and folds it over itself to make it fit into the cell nucleus. By doing this your breaking down the histones which increased the DNA's length.

[Sticky1254]

last I checked sodium doesn't do the same thing as polymerase, so no.

[LovecraftGirl777]

I agree. I get a bit nervous if I have to talk to a big crowd.

[LovecraftGirl777]

Have you ever considered teaching science? You would be very good at it! :)

[bionerd23]

i'm not sure about that. :P

it's quite different to talk to a crowd of people than to just talk to a little camera.

[marianile]

@LovecraftGirl777

she may be already..

[sakuramomijisan]

hey! are u a dr? or ur BS BIO? wow i love u bcoz of ur video i am loving my Bio 1 and 2 and 150!

[atomicnerd7334]

easy, dna disolves in water, not in alchohol. it falls to the bottom. i am not sure what the washing up liquid is for, the salt helps disolve the dna

[bionerd23]

afaik the tensides in the washing up liquid destroy the cell's barrier / skin... in some sort of the same way it destroys oil bubbles, i guess. :-)

[nerd13377]

lol i heard mario at 8:54

[habbopassload]

* Mario theme song *

Goddamit!

[AoIministrator]

HOT!

[digitalimpulse]

Is this why they swab your mouth for DNA testing?.. to do this?

[bionerd23]

exactly :-)

[LechuCzechu]

Its funny that alcohol, soap and salt were know to humans thousands of years ago, even the first microscopes were made about 300-400 years ago. But not until 1950's nobody realized that this simple method is effective. Very cool.

[zmandt1993]

what you are seaing is not a single strand of DNA but rather meny strands. posibly hundreds of fousends of strands and cell membrane. are all twisted up in to something like a rope.

[bionerd23]

yeah, i think you could be right on that.

i will try and do some more advanced microscopy like giemsa staining and dark-field microscoping once i have the time, and find out more. giemsa staining allows to see the nucleus of a cell, and i could then make the cells explode due to osmosis, and see what actually happens / is visible from the cell's core.

[Recklesssennin]

actually its more like a ladder

[arequipa1]

whoa freaky!!!!!!

[atomicnerd7334]

iam nerdy and ill explain.

you are trying to preciptitate the dna in isopropanol .

P.S i am pyrochemistry

[TheCynicalAtheist]

Anybody have any idea how this works?

Im thinking its probably something to do with the cells rupturing because of the salt water, and then the contents rising up into the alcohol, but i have no idea.

Atoms on the DNA molecule bond to the sodium chloride? and become hydrophobic?

Im going to try this myself when i have a microscope though.

Was allways under the impression you can only see DNA with an electron Microscope,

Great Video.

[bionerd23]

yeah, i suppose the cells rupture because of osmosis. my guess is that the dish soap then keeps apart the liquid and DNA / fragments, same as it keeps apart oil and water... if you add a drop of dish soap to oily water, the oil will move away from the site. this has to do with tensides, but i am uninformed of details... i'm just guessing it does something similar in my experiment. i dont know why exactly they are attracted to the alcohol, either; i am more of a physicist than a chemist... :-(

[bionerd23]

i think sodium chloride bonds to everything when a solution dries... the crystals were growing on the plain microscope slide as well. ;-)

as to my knowledge and experience, you need an electron microscope to actually see chromosomes on DNA, but you can obviously see DNA as a single strand even with a normal light microscope.

[bionerd23]

actually it just came to my mind that probably the tensides within dish soap just help the osmosis / rupture of the cell, or even more like, the cell's core. so, they make it easier for the cell as well as it's core to 'explode' and thus, release the DNA. the isopropanol is probably the agent that really seperates the DNA from the rest of the solution.

just guessing here though, as i said.

[bionerd23]

d'oh, double strand it's meant to read above, sorry... surely we have DS-DNA, not SS-DNA.

[doktorfuture]

marry me! you're awesome!!!

[Chaoschemiker]

There are only 10 kinds of people: Those who understand binary, and those who not. :D

Der Einführungs-Biochemie-Versuch schlechthin aber ohne Mikroskop irgendwie "nutzlos".

Ohne hat man nur diesen weißen Pansch.

Schön, unter eigener Aufopferung, gemacht :D

[bionerd23]

wo ist das most significant bit? bzw. heisst das jetzt "there are 1 kinds of people" oder "there are 2 kinds of people"?! :P ^_^

aber danke. ;-)

[tnzfki]

super mario

i like your attitude towards nature

[bionerd23]

lol that was just my mobile phone going off... ;-)

[idotechno]

This is so cool. I wish I had a microscope to try this out. I'd never take my own sample of blood, so it's good that there is another way to try this. So awesome.

[bionerd23]

yeah, maybe i should retry without filtering, actually... good point.

[TribalWarsTube]

that blood kill vamires :D lol poisin from soap

[virozir]

What were those thin black strands near your DNA?

[bionerd23]

i'm not sure what you mean? but around my DNA, you could see sodium chloride crystals forming from dehydration... they look much like fern plants.

[corvardus]

I think it was some hairs across the lens or something. I believe he might be talking about that which you can see clearly @ 8:03

[bionerd23]

oh that stuff! yeah that indeed is hair / dust on the side of my microscope... i need to clean that out with pressurized air or something some day. -_-

[virozir]

I remember doing this in high school bio class... but we didn't use our own blood :O

[bionerd23]

yeah, at school, you mostly use tomatoes and stuff to do this experiment.

[jason959595]

lol she had the mario theme on her phone

[10mintwo]

wow! that was fascinating!!! I had no idea you could separate out macroscopic quantities like that with only mouth rinsing! I'm DEFINITELY trying this with my cum to see if I can extract more or less than from saliva. Is that gross? oh well, I don't care! science!! wo0t!

[bionerd23]

if you have a microscope, look at your sperm through it, it's awesome!

but getting the DNA from it should work fine, too, i'd assume - damn, i envy you for being a man now. ;-)

and yes, for criminal records, only a small swap is taken from one cheek, too - that's by far sufficient to isolate a large enough amount of DNA. you wouldnt BELIEVE how many cells you are losing all the time, e.g. from your skin...

[fakenphoney]

that's not DNA...

[bionerd23]

right. what is it, then? :-P

[amethyst8teen]

Will denatured alcohol destroy the DNA?

[bionerd23]

hmm, i assume it wouldnt, as isopropanol doesnt, either - but i'm not enough of a chemist to know that for sure.

[alin0steglinski0]

and you can see 8:54 god damn it! LOL

[alin0steglinski0]

btw, COMPLETELY safe? I am not sure how safe messing with needles is :P

[bionerd23]

i was referring to the actual 'working' procedure, that is gargling with salty water, as i mentioned later in the video. :-)

[TribalWarsTube]

well if u think needles aretn safe ur weird

me: needles ok

bullets not ok

[alin0steglinski0]

bullets are not ok

needles are SEMI safe thats why i said its not COMPLETELY safe. what if a person with haemophelia watches the video and follows it. or someone like me with bad fine motor control (damn you neurological disease) having a gun in my hand is worse, having just a needle i could blow quite a few veins before i get a good sample.

[bionerd23]

errr... if somebody watches this video and attempts to follow it, i guess they have had quite a few wounds in their life already and know if they have haemophilia or not... i know i hurt myself up to the point of bleeding way before i was even old enough to understand such things like mentioned in this video. :-P

[TribalWarsTube]

I am very sorry to hear about your disease. I was talking about myself. I have steady enough hands to inject myself with a needle as done in the video, and without it hurting too much

[alin0steglinski0]

thanks for the apology, this is one reason why i DONT go on youtube often or comment on videos because a huge percentage of people on youtube are pure assholes who dont even apologize. so i really hold respect for you because you are one of the few people on youtube who have respect for other people especially people who are dying of unknown cause

[TribalWarsTube]

No need to thank me. Just more people should care. Anyways god bless

[amethyst8teen]

Can this method work for any living tissue?

[bionerd23]

well, the procedure would kill whatever is living, as to isolate DNA, you need to break up the cells and expose the inside of the nucleus, and that is equal to killing the organism.

[amethyst8teen]

I have to try this! Great video! I loved it! What kind of microscope do you have?

[bionerd23]

it's a BRESSER BIOLUX NG. costs about $100 at max. i think... max. resolution is 1024x plus a zoom lens, so you can see blood cells quite well, and you can even see e.g. sperm VERY well. as i dont really know where to get a sperm sample, i cant do a video about that, but i'd love to... darn. i should just ask some random guy to wank into a cup.

[alin0steglinski0]

go hire someone say heres 5 bucks go wank into a cup for me *point at stall

[alin0steglinski0]

wow, able to draw your own blood!!!

[bionerd23]

it's not hard... its just that most people are scared to make themselves bleed / prick into their own veins, but the procedure itself is very easy to accomplish.

[alin0steglinski0]

easy to accomplish ehhh yes if you take some time to learn but me having fine motor control issues i would blow my veins so much id be like *prick* dDAMN!!! *prick* DAMN!! *prick* DAMN, THE HELL WITH THIS, I'll get my doctor to hand a sample over

[clony101]

where did you get the strap thing

[bionerd23]

medical supply store.

[alin0steglinski0]

need some... i have loads of rubber strap things ive collected over the years at the hospital. i also have a few of the reusable strap type ones. LOL. Email me so we could talk about mailing em ROFL

[clony101]

could you get the blood from a resently existing cut or scab

[bionerd23]

yeah, but the quantity will be smaller, obviously.

[clony101]

i dont like needles at all thats why

[clony101]

damn i could never get a blood sample by my self

[iPervy]

I'd like a sample ;D

[bionerd23]

hmm maybe i should really start selling it. ^_^

single hair $5, spit solution $30, extracted DNA $50. lol

[Dividend]

Binary watch?

[bionerd23]

yesh!

[pyrochemistry]

hey, since Im nerdy, ill explain how it works.

You are concentrating the deoxyribosenucleic acid and is insouble in propanol.

please suscribe or be my freind

[bionerd23]

well, why does it move from the solution into the isopropanol though? :)

[klutz22]

LMFAO

[Dubaifreak]

ebay...lol....DNA at sale! hehehe

[polonium9]

i have the same ringtone!!!!!!! go mario!!!!! check that DNA for radioactivity if you like!!

[Dubaifreak]

You are awesome!!! lol

[drumbum99]

is that a binary watch =P?

[bionerd23]

lol nicely spotted, yes indeed it is!

[drumbum99]

haha iv wanted one of those for ages

alas theyre a bit expensive for me at the moment =P

[elmeister4evayoknow]

fake?

[bionerd23]

just google for it, you will find out it's not fake. or just try it yourself. :-)

[JaksProductions]

nice video anyways :)

[bionerd23]

i hate this "1st" stuff shit, so i deleted it. sorry, nothing against you, i just hate it cuz it's useless. :P

[JaksProductions]

hihi okay,I'll never do it again!:)