Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?
@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.
Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?
MalagaJolu 3 months ago
Holding the pipette vertical and pre-rinsing the tip will help you get more accurate results and reduce the variation between wells.
MonsieurLavabo 3 months ago
the coating buffer allways have to be 0.1 M Carbonate pH 9?
MalagaJolu 3 months ago
@MalagaJolu No, it really depends on the assay you are doing. For example it can be an antibody in Blocker Casein/PBS.
MonsieurLavabo 3 months ago
Will the coated antigen in the plate being shaked out if you hit the plate against the table so vigorously??
0102murphy 4 months ago
@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.
MonsieurLavabo 3 months ago
We don't have name for background music but we like it as well. Thanks for your support!
ABNOVA1 1 year ago
Haha, weird question but what is this song called? Its really nice! Excellent video series on molecular techniques by the way!
PreMedReview 1 year ago