Protein Synthesis, Translation (1)

[pinkpillow16]

the visual and explanation of your video was very simplistic and helped a lot. Thanks

[bscbiology1]

it lack information..anyway it was helpful somehow

[danthesoccerfan77]

forgot the E site, but i still understood more than our teacher's explanation!

[DunkItaly]

thank you so much.

[saharchodhary]

nice explaination ... !!

[NYtassu]

Look at that ribosome hand moving along that mRNA pole.

[NYtassu]

Wait, isnt there an E site on the large subunit?

[szeretetesbeke]

@NYtassu Yes, there is. The E site is where the discharged tRNAs leave the ribosome after they've donated their amino acid. In the video, the P site was to the left of the A site on the ribosome. You can think of the E site as being to the left of the P site. So instead of being released in the P site as they were in this video, the tRNAs leave through a third site, the E site. Hope that helped :)

[Tl82T]

that is only the synthesis of polypeptide not protein!

[nkaohmong]

I hope you guys know.this is old.. they don't even talk about the e site?!?!

[BeauLeeman]

"why o why haven't instructional videos replaced text books"

Actually, the Internet has replaced textbooks, as well as the need for class room study. While live lectures are good, at least if exchanges are allowed, you can get the same and MORE info online.

In fact, more and more schools are using it for student instruction, with only occasional classroom meetings.

If properly disciplined, one could actually glean all needed data, including post doc work on their own.

No diplomas tho ...

[shanefarrell]

awesome!

[jmcgee10]

thank you very helpful

[djsanchez718]

Thanks, we liked this!

[drshankar]

beginning of the video: "transul-translation..."

[jenhappygirl954]

Very well explained! This is so much better than many of the medical biochemistry books I have read. thank you!

[ngobmx]

@jenhappygirl954 lies!!!

this is almost an irrelevant video, completely misses the entire initiation complex

probably misses more but stoped watching ti

[6YourPrettyFace9]

The larger ribosome segment looks like a baseball glove...

[khoinguyenmath]

How easy to remember this process! thanks so much ^^

[ParanoiaDestroyah]

What happens to the mRNA after the process?

[d4ark]

@ParanoiaDestroyah What do you mean? It's turned into the protein, since the mRNA only consits of the base-triplets that are needed for a certain protein. The tRNA-molecules have more triplets, so they can be 'reused', but the mRNA completely ends up in the protein/peptide.

[ParanoiaDestroyah]

The monotone makes me sleep zzzzzzzzzzzzzzzzzzzzzzzzzzzzzz­zzzZZZZZZZZZZZZZZZZZZZ

[deadpool93x]

what happens to the mRNA when the process is finished?

[melbourneopera]

Hmm, 1st time to heard about "release fastor" to terminate the polypeptide chain packaging...Thanks for the introduction

[flynnuhboi]

You'd think a 100 dollar book would put it in a language you would understand but a free video did what a 100 dollar book couldn't do

[zamus2004]

This video made sense than these dumb expensive books!!. Thank u!

[fumfulapenguin]

this is much easier than looking some dumb images in the book

[LydLyd95]

@kiro1990122 : ahaa still dont get that joke :p .

[therealjordiano]

AHH !! thank you, finally O_O non of the book descriptions make any sense

[CODBlackOpsTopTen]

this animation stuttered at 0:00

[GeekOfNature786]

this shit better not come up in my exam!!!

[ABomm77]

My teacher was like we dont have enough time for this video so you can watch it on your own. This 2:16 was a lot easier to understand than her 10 minute explication w/ arrows

[LydLyd95]

i still dont understand.! :( .

[kiro1990122]

@LydLyd95 then join the army lol  jk

[mephesh]

What facilitates the tRNA to arrive in the correct order to match the mRNA codon sequences?

[mallllyyy]

this helped me soo much!

[ninjaartist33]

What happens to the tRNA after bond is formed? Does it go off an find another amino acid?

[Folypeelarks]

@ninjaartist33 Yes.Actually before every translation the tRNA-s are allready connected to the amino acids.This connection is made by the enzymes aminoacyl tRNA - synthases (that's why the site where tRNA enters first is called aminoacyl site or aminoacyl entrance).When tRNA leaves off the ribosome it'll be recharged with amino acid by such aminoacyl-tRNA synthase.

[Fussballgott1993]

Great video! I finally got it. Thanks for upload.

[155nate]

Excellent Video!! Great Explanation. Thank you for your contribution to the confused college kid's life.

[BallerBeenz]

damn that mrna is probably horny as hell after the ribosome subunits jacking it off all the way down the chain. thats why it reaches its climax and busts with the stop codon.

[12sweta]

i swear I sat in 3 hours of lecture and didn't understand anything and this 2:15 explained a lot. thank you very muchooo

[YourGdiGuide10]

An animation is worth a 1000 words..

[Holbeinisscheisse]

in 2 minutes i understood more than our teacher told us in 5 weeks

[janicetiaaa]

@Holbeinisscheisse LOL same here!

[preetypal]

This is really good

[objextr]

Time to add E site



[lainemichelleallen]

thank u for simplifying it!

[metfan89]

probably a stupid question but i know very litle about bio, what happens to the mRNA after protein synthesis is complete?

[MissLilium]

@metfan89 can be reused

[WorldCollections]

@metfan89 I once asked the same question. I was told mRNA is "dissolved" by enzymes within the cytoplasm.

[cbernier3]

@WorldCollections You mean hydrolyzed, not dissolved.

[mephesh]

@metfan89 mRNA is protected by a cap and tail. The length of the tail defines the amount of times it can be transcribed. Each transcription shortens the tail. When the tail is sufficiently shortened the tail and cap are removed from the mRNA and the mRNA is degraded.

[traductorvlc]

Good video. Check out for traduccion aleman espanol its a good translation link.

[dontspeak11]

According to a few sources I looked at, the ribosome moves 3 nucleotides along the mRNA after peptidyl transferase forms the bond in the A site. In this animation, the mRNA moves along the ribosome.

Very helpful, overall.

[ELkORji]

very useful, nice.

[SantoRedentor]

There are 3 sites in the ribosome, A, P and E . Not only 2

[dalooloo28]

it looks like chicken

[1579sean]

this really makes sense............lacking one r two bt what the hell.the text didnt highlight most of what i saw here

[musicalyam]

I don't understand where the transfer RNA comes from and where it gets its anticodon and corresponding amino acid. How are they directed to the A site?

[reddeviljojoayan]

amazing incredible!!!!!!!!! God this really helped! the text books are full of useless complicating shit i really wished the books explained so easily

[youyesyay]

Beautifully and clearly explained! Thank you :)

[gilmourgirl74]

Brilliant video!

[zuppah]

Pretty awesome video, just read all this stuff but didn't imagine it at all being like this. I get it now though.

[batthatisbat]

Which moves, the mRNA molecule or the ribosome itself?

[jbowenatcg]

@batthatisbat the ribosome itself moves along the mRNA

[Selaaaab]

Very nice

[fightingnate]

what does the E site do?

[eragrim]

fantastic

[gottspieler]

why o why haven't instructional videos replaced text books for bio, chem and history classes yet? We'd all be smarter if the information were presented so clearly to us

[1aaronaaron1]

I don't journals will ever be replaced so it's still important that you are able to learn from reading a textbook. Most textbooks have websites with good animations and stuff...

[aks767676]

How the heck did scientist discover this stuff. Its amazing. Like a factory making stuff.

[Spetsop]

If you think this is interesting, check out this video. It's an animation of some of the things that go inside the cell (from Harvard university), it's really interesting! If you haven't seen it yet, you'll love it for sure :)

watch?v=BVvvx5HGpLg

and this one is the same animation but explaining it in details:

watch?v=fZZ3DD_tV9k&feature=re­lated

Enjoy and all the best :)

P.S. This video is really good at explaining the process!

[djnross]

Think about this:how would you even begin to explain this to Charles Darwin?Or for that matter----Gregor Mendel,who suspected something like genes being responsible for all heredity?

[saailaway]

sweet thanx

[grThetrojan01gr]

thanks, it helped me a lot!

[DaisyDenise11]

Wayyy better than any book description.

[pingu121094]

@DaisyDenise11 agreed

[yourleaderslie]

thats an amazing video, thanks for posting it!

[Xelastic]

a picture can replace a 1000 words,

a movie clip can save some nerve cells :)

thank you for the post!

[linkobsessor247]

I understand now

[SereneSoul5]

ohhhhhhhhhhhhh now i get it.

[nycnazifa212]

lol i was thinking the same thing.

[00300odyssey]

very good

[punkito9]

ummm no what about the e site.

[pinkmango00]

this video is missing the E site of the ribosome... it doesn't just exit the ribosome on its own... it moves into the E site which in turn makes it leave! :)

[dalooloo28]

@pinkmango00 ya i was wondering that as well

[ab0000dy1988]

@pinkmango00 yeah you're right , some books discuss an E site (ejected) but for the sake of simplicity just P (peptidyl) and A (aminoacyl) sites are consider in many references .

[Solarisx06]

@pinkmango00 The E-site is not present in eukaryotic ribosomes, only prokaryotic/bacterial.

[DisturbedLunchBox]

@pinkmango00 Actually, only prokaryotic ribosomes have an E site. Eukaryotic ribosomes do not have them, and the uncharged tRNA leaves directly from the P site.

[meka337]

@pinkmango00 iknow right i know that much lol

[txlnghrngrl2009]

@pinkmango00 The E site is only needed for prokaryote translation. For eukaryote translation that uses the 40S and 60S ribosomal subunit, only the A & P site are needed. 

[josefgiven]

Great video. So what happens to the tRNA once its amino acid has been assembled onto the polypeptide?

[PegasoltaEclair]

It leaves XD. And goes to pick up another amino acid.

[cw505]

can't we just say "because god did it"??

it would make everything much easier in life.- missisipi, alabama, louisiana, etc...

[angela523523]

yea ..

[poofyluv159]

wow this is so funn :)

[abbseyrawr]

interesting. not.

[ebingen]

Comprehensible, thanks.

[dreamslayer779]

there are A LOT of things in this video that were believed to be true like 15 years ago... but they are not

[JKM12988]

I thought the ribosome moved down the mRNA, not the mRNA moving through the ribosome?

[Unloadedx5]

Your looking at it differently. Ether way it wouldn't make a difference in the video.

[tsferg]

I know brent spiner when i hear him. Good narration Data.

[NTUCSBY]

The ribosome looks like doraemon's hands

[sandraider56]

holy crap that simplifies things so much! i couldnt find a decent picture explaining this in any books.

[ruthlessghost]

WHAT ??????????

[Animechiongster]

nice video

[Kieranmusic89]

Great vid. Cheers

[howlongcantheusernam]

Binding an amino acid at 3' end of tRNA captures 2 high energy bonds of an ATP molecule that will then be released during a peptide bond formation. This stuff doesn't happen magically on its own.

[PinkooRani]

Hey, I was wondering if it was safe to say that the ribosome reads the mRNA codons in a 5'-3' direction. If that's the case, can I assume that the tRNA is complementary, with it's 3' end "corresponding" to the N-terminus of the aa that it is carrying and it's 5' end corresponding to the C-terminus? I'd really appreciate the help!

[compuhelp101]

lol yeah, i have the regents today and i understand it now

[Karaliese123]

THANK YOU!

[laraineb]

Give the exit site some love!

[Forumsnobordz]

hmm... too simple for biochem lol

[jeedizzle]

GREAT VIDEO!!! Im in college biology and damn this was good. Thanks!

[microtech23]

i was confused by the book for like an hour...this explained it in 2 min!!!!!! thanks a million

[pibbleswithjeff]

this is hella useful

the book is a piece of crap when it comes to visuals

[Element6tight]

i am only 12 and we are learing this, this makes it so much easier, manly i better at learning with vidioes. =)

[Pinoynakungpinoy]

hmm... what about the E-site?

[pedant0]

That's the exit site that they forgot about it. LOL. It's not that important though.

[gaarate]

I think that only Prokariota own E-site. That may be the explanation.

[alyssa0taylor]

there are 3 binding sites for the tRNA on the ribosome,

the third is known as the E site.

:)

[ammo931]

what is the purpose of the E site?

[alyssa0taylor]

In simple terms, before the tRNA leave the ribosome they are bound to the E site :)

[uadshfkhdsf]

you are the man it was hard to understand with my brain and my book

[harshjhaiiita]

thanks..

[Levirobertson]

this rocks!!!

[CedricBahde]

grüße an den bio Gk!

[CedricBahde]

simply the best! <3

[josie1so1cute]

video not available?? is it my computer is it just simply not available?? i got a report and i so0 badly need to watch this!!

[pepefoot]

refresh the page

[Brokengaze]

Isn't the codon broken off once it reaches a terminator set? I am kind of oblivious to the end...

[grim0033]

the codon doesnt break off at all the codon(s) are located on the mRNA. The tRNA's (which carry a 3 base sequence known as the anti codon) break off once they have passed through the p site and the peptide bond of its amino acid has formed with the rest of the polymer. The stop codon prevents any more tRNA entering the ribosome (A site). Through catalytic processes by some enzymes and energy provided by GTP hydrolysis, the ribosomes then split and leave the mRNA

[Medguy83]

nice. of course the whole E site thing is missing, but then again there's a lot of other extra info missing as well; EF2, EF1A, ribozymes, etc. good overview though!

[txcapp]

omg helped me so much! thank you!

[snipoppers16]

This is great, but it omits the E site. The tRNA doesn't just disappear, it is shuffled off to a third site to the left of the P site.

[gojaysgorush]

yeah I learned it with 3 sites as well

[osterhornum]

what is this ??????

[s0ulshine]

Videos like this work absolute WONDERS for understanding simple cell bio... much love for whoever made this!

[nemeczek67]

Simple yet very informative

[GeneralBirdMan]

thank you, this video is just what I needed because my teacher didn't do a very good job explaining it in class.

[kawada2998]

thanks ! ive got exams tommorrow, and this has helped

[anakdekan]

I UNDERSTAND NOW!!! THANK YOU!! This video really helps me!! Thank you sooooo much! I LOVE whoever made this video!!

[GUNSGIRL87]

Thank you! That helped alot

[MimisaW]

thanks for this video

it´s fantastic

[yberrios81]

Excellent!!

[hhoollyywoodd]

wow i actually understand it now! yaya

[satanversusjesus]

Needs the E (exit site) E.P.A.

not just P.A.

[elevatoor]

prejebeno

[dirtydogpig]

this is not accurate, the transfer RNA's are ratched from the A site to the P site to the E site and are positioned in a number of inbetween hybrid states carried out by elongation factors like EF1alpha and EF2. they don't just fall off from the A site..

[f14chris]

You're right about the E site. The video is just like an overview of the process. U learn more about the process in a genetics class.

[zsuzsiiii]

thanx :)

[lin1992912]

Thank you very much!! I understood really well!!! a lot easier than textbook

[bengaliballa]

Yo is translation the same thing as Protein synthesis?

[iota1122]

If no one has answered your question on translation being the same as P.synthesis, it is not. Translation provides the components necessary for protein synthesis.

[f14chris]

Usually when people say protein synthesis they usually mean transcription and translation combined. Translation is a more specific term while protein synthesis is a more general term

[iota1122]

I see your point, I was not trying to be an ass or anything, but I study genetics and work with genetic problems in detail. I did not know where that person was having problems in the central Dogma, so I did not know how much info to give or not give. Guessing you are aware of how many steps there are between the RNA leaving the nucleus (if eukaryote) and actually forming a protein.

[magick205]

One thing that I always have trouble with is understanding the mechanisms behind editing the exons and introns out of the primary transcript. Is anyone familiar with a vid that might show that in a little more detail?

[iota1122]

I do not know of any video, but some of the mechanisms are: intron branch point, lariat, 3'-OH group (adenine)linking 5', snRNA, and small nuclear ribonucleoproteins or "snurps". You might try to go online and see if you can find a Micro biology or Genetics texbook that has a CD study guide,they usually have great videos on them. I would be happy to help you with any of your questions as well,if it something I understand and can relay back.

[jeet0131]

great video.

[as89han]

whoa thanks man amazinly great....hope it works out for my exam tomorrow

[pwalkkwalp]

you forgot the e site

[djibriltheguru]

there is a set of 3 proteins if1 if2 if3,the initiation factors rquired for the process. if3 prevents premature binding of 30s & 50s subunits

[djibriltheguru]

great video. thank a lot

[arw353]

the best translation vid i've seen, AUG is both start codon for translation and for transcription?

Is the first tRNA with meth aslso known as an initiation factor?

[chipazoid]

No? first tRNA is brought by IF2. Different proteins.

[pkranged105]

Woah, this is great, all i intended to know man!!!!, cool