mRNA purification is does really a necessary process for quantitative real-time assays? I ve try to extract and purify mRNA from pigmented plant tissues in order to obtain the first strand of cDNA. But i have the question if this step of purification it is really necessary.
Ok...thank's
edmonte3 2 years ago
mRNA purification is does really a necessary process for quantitative real-time assays? I ve try to extract and purify mRNA from pigmented plant tissues in order to obtain the first strand of cDNA. But i have the question if this step of purification it is really necessary.
edmonte3 2 years ago
Nope, it's not necessary for qPCR.
kubu420 2 years ago