@thesystemsfailed: The neuron appears flat (and 'squashed) because it was grown in a culture dish; neurons grown in dishes tend to flatten out against the dish and substrate to which they adhere. The true '3D' in vivo context is, unfortunately, lost in cell culture.
These movies are made from pseudo-confocal image stacks acquired on a Leica DMIRB-E inverted fluorescence microscope. Deconvolution and reconstruction were performed using the image acquisition and analysis software package Slidebook 4.0 (Intelligent Imaging Innovations, Inc., Denver CO). DAPI was used to label nuclei.
"TO ALL PEOPLE WHO ARE READING THIS : DOSE THE ELECTRICITY OF BRAIN CAN BE HACKED VIA SOME TECHNOLOGY? Can ANY Microwaves or ELF Waves Manipulate the Electricity of the brain?
My Clear Question : Do some people Reading OUR MINDS And manipulating our subconscious regions of mind-brain via having the electricity of our brain in thier computers?
Flat and squashed.
bin4z 1 year ago
Is that really fully 3d? I didn't think they structured along such a flat plane. Its interesting if they are, I guess it would be far more organized.
thesystemsfailed 1 year ago
@thesystemsfailed - but less flexible... read: "plasticity"
tzeqin 1 year ago
@thesystemsfailed: The neuron appears flat (and 'squashed) because it was grown in a culture dish; neurons grown in dishes tend to flatten out against the dish and substrate to which they adhere. The true '3D' in vivo context is, unfortunately, lost in cell culture.
DocBones 10 months ago
Was that live or fixed?
cutiephotographer 1 year ago
@cutiephotographer:
The hippocampal neurons were fixed. Live would have been really, really nice, but . . . well . . . you can't have everything! ;-)
Thanks for the query!
DocBones 1 year ago
These movies are made from pseudo-confocal image stacks acquired on a Leica DMIRB-E inverted fluorescence microscope. Deconvolution and reconstruction were performed using the image acquisition and analysis software package Slidebook 4.0 (Intelligent Imaging Innovations, Inc., Denver CO). DAPI was used to label nuclei.
DocBones 1 year ago
This has been flagged as spam show
"TO ALL PEOPLE WHO ARE READING THIS : DOSE THE ELECTRICITY OF BRAIN CAN BE HACKED VIA SOME TECHNOLOGY? Can ANY Microwaves or ELF Waves Manipulate the Electricity of the brain?
My Clear Question : Do some people Reading OUR MINDS And manipulating our subconscious regions of mind-brain via having the electricity of our brain in thier computers?
I hope for Answers!"
mrelectron111 2 years ago
C'est fait par microscope à déconvolution?
simsima34 3 years ago