Added: 4 years ago
From: DocBones
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  • Flat and squashed.

  • Is that really fully 3d? I didn't think they structured along such a flat plane. Its interesting if they are, I guess it would be far more organized.

  • @thesystemsfailed - but less flexible... read: "plasticity"

  • @thesystemsfailed: The neuron appears flat (and 'squashed) because it was grown in a culture dish; neurons grown in dishes tend to flatten out against the dish and substrate to which they adhere. The true '3D' in vivo context is, unfortunately, lost in cell culture.

  • Was that live or fixed?

  • @cutiephotographer:

    The hippocampal neurons were fixed. Live would have been really, really nice, but . . . well . . . you can't have everything! ;-)

    Thanks for the query!

  • These movies are made from pseudo-confocal image stacks acquired on a Leica DMIRB-E inverted fluorescence microscope. Deconvolution and reconstruction were performed using the image acquisition and analysis software package Slidebook 4.0 (Intelligent Imaging Innovations, Inc., Denver CO). DAPI was used to label nuclei.

  • C'est fait par microscope à déconvolution?

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