Glycolysis

[simone2254]

thank u!!!!!!!!!!!!!!!!!!!!



[everg14]

I don't get why these are happening...I wish there was some explanation.

[jisaid08]

@everg14 It was difficult to explain and put out the entire process in the 10 minutes youtube allows. Next time I will try to cut up the video into sections.

[halfpup]

i'm half way through the video but so far all i see is regurgitation on to the board with no explanation...

[hugeworld28]

thaaaaaank u :)

[planetlexicon]

Thank you for your help! I have to memorize this for my Medical Biochemistry class.

[jisaid08]

@planetlexicon Glad I could help you. :)

[TheFatgiraffe]

soooooo confused..... but im in middle school so i guess its ok

[TheFatgiraffe]

soooooo confused..... but im in middle school so i guess its ok

[LetsWangChung]

ths is junk , anyone can just read this in a book

[jisaid08]

@LetsWangChung Well 33 thousand people have watched it and I've had a lot of thanks for posting it. So if you don't like it...Don't watch it. Thanks so much.

[acute00]

Thanx so much ,, so clear 

[kickingtkdgirl]

aldoase actually cleaves the molecule into 3 carbon fragments, GAP and DHAP (which is converted to GAP via triose phosphate isomerase and the laws of mass action).

[CaptainJenna7]

thanks very much, you're awesome.

[DaK1LL]

Holy Shizer! i had no idea glycolysis was so complicated...

[preeti41]

thank you so much. it was very helpful.

[bmarieGbolivar]

can u teach me again with this? i really need your help

[Cervier24]

wrong...

[bloklover]

HAHA, you say you are going to do it by the book but you forget dihydroxyacetone phosphate.. AND its not phosphophenolpyruvate :p but phosphoenolpyruvate.. that is the enol form of pyruvate plus a phosphate group, hence the name ;)

[redefinel0ve]

YOU'RE AMAZINNGGG!! this helped sooo much

[ariftalpur]

In this whole breakdown we have got 10 ATPs, as we have 2NADH and 4 ATP and here we also know that 1NADH is equal to 3 ATPs so we have 2NADH=6ATPs.

[crystal2nero]

the forth reaction is the cleavage of fructose 1,6- bisphosphate into Dihydroxyacetone-phosphate and glyceraldehyde3-phosphate. the fifth reaction should be the isomerization of dihydroxyacetonephosphate further to glyceraldehyde 3-phosphate in the presence of triose phosphate isomerase.. i'm not so sure.. But fenks all the same for the video.. it really helped me.

[uniterscron]

You may want to mention the little guy, dihydroxyacetone phosphate as another product of fructose 1,6-bisphosphate catalyzed by aldolase.

[diniefofinie]

i've always wanted a white-board

*sniff*

[maccaddy17]

dude i tink on the overall 2ATP is produced.

bcuz at 1st its 2ATP is used(-2ATP)

then frm G3P to pyruvate 2ATP is produced, and that whole reaction is doubled ryte??

so basically 4ATP-2ATP=2ATP.

[TheExPlOiTeDOne619]

you're 100% correct.

[cheskabelle]

my professor explained to me that is phosphoenolpyruvate (PEP) not phosphophenolpyruvate (PPP)

[uniterscron]

it is phosphoENOLpyruvate, why would you want a phenol group in your energy-making stuff?

[cheskabelle]

wait, it's phosphophenolpyruvate or phosphoenolpyruvate.......?

[mefranco22]

It is not a reversible reaction going from 1,3 bisphosphoglycerate to 3-phosphoglycerate.

[timmyfourtwenty]

didnt read through all the comments, so it may have been mentioned already, but how come you dont mention dihydroxyacetone phosphate? I know its short lived before it becomes glyceraldehyde 3 phosphate, but worth mentioning instead of just saying that fructose 1,6-bisphophate becomes G3P x2.

[kpaul00]

Yeah. The aldolase reaction splits Fructose 1.6 bisphosphotate into dihydroxyacetone phosphate and glyceraldehyde 3 phosphate

[vsm48]

you missed the triosephosphate isomerase (aldehyde to ketone) but overall it's ok!

[glljes]

Is he wearing a snuggie?

[espears721]

Yeah aldolase cleaves it definately. Maybe he needs more practice.

[ruialves52]

I think you might have said something wrong...you say that glycoaldehyde 3 phosohodehydrogenase has cleaved the 6 carbon molecule into two molecules of 3 carbon. but that cleavage was made earlier by aldolase, not by this enzime. but I'm not sure of this

[omer0alvi]

yes... ruialves52 is right .. the enzyme which cleaves the 6 carbon mole , that is fructose 1-6 bisphosphate into 2 3 carbon molecules, called phosphoglyceraldehyde is called "FRUCTOSE 1-6 BISPHOSPHATE ALDOLASE" and not "glycoaldehyde 3 phosphate dehydrogenase. ... glycoaldehyde 3 phosphate dehydrogenase. is the enzyme which adds another phosphate group to the two 3-carbon molecules.

[aysoreferee459]

Thank you so much. I have a test on glycolsyis tomorrow and you just made things a little easier for me.

[Tubengirl]

this was great, i have a bio exam tomorrow!! it would be helpful if at the end you went over all of the products.

[mikeflsara]

What about the anolase spliting the bisphosphate into two 3 carbon units glyceraldehyde with 3 phosphates and dihydroxylacetate with 1 phosphate? You mention glyceraldegyde but not dihydroxlacetate.

(abbreviation) PEP-PhosphoEnolPyruvate

[sangretaina1986]

Aldolase does catalyze the reaction 1,6 FBP to DHAP and G3P, he probably omitted since triose phosphate isomerase will isomerize DHAP to G3P (since only G3P can continue). And glyceraldehyde 3 Phosphate doesn't have 3 phosphates, the number refers to the carbon that the phosphate group is attached to. Both DHAP (DihydroxyaceTONE phosphate) and G3P have one phosphate group each, DHAP has one at the 1 carbon and G3P is phosphorylated at the 3 carbon.

[doctordrori]

hey, thanks for the effort :-)

is it possible that you skipped Triosephosphate isomerase step?

[ItsWhatever24]

the end product is Phosphoenolpyruvate (PEP) (x2)NOT phosphoPHENOLpyruvate. Dont get this wrong people

[ItsWhatever24]

sorry im referring to the second last step (2-PG --> PEP)

[jisaid08]

Sorry about that, I didn't realize I had said phenol and not enol.

[FeastsandFires]

Thank you so much for this! Wow best video on YouTube. I understand it so much more now!

[Gr33nBlaze360]

wow this is confusing lol but great work

[kainoaroy]

Thank you so much, this is greatly appreciated...seeing it written out like what you did on the white board made things much more clear to me...Keep up the great work.

Cheers

[drkangel624]

thanks.. its completely understandable now... in class i was "huh"... thanks again for the vid

[met0xy]

In the second to last step, it's phosphoenol pyruvate not phpsphophenol pyruvate.

[Mheatherette]

if it wasnt for the fact that I had already been familiar with this cycle I would have been totally lost ...too detailed shouldof generalized first

[chenghuihsieh]

thank you. I love the video. I am looking forward for your next video

[amm23ka]

Oooooh very helpful :)

thankz!

[ReviRevInfinity]

Thanks man, it's always good to hear a fresh perspective on these types of things.

[bursaursa]

hey man you missed out on dihydroxyacetone phosphate. it is produced from fruc1,6-bisposphate along with gliceraldehyde 3-P. It is then converted to glyceraldehyde 3-P with isomerase.

but sience you are a fan of biochem i guess you already know that:)

greetings form slovenia

[silyangpilak]

thanks man!

[jumboboy]

that was extremely helpful, thank you

[CodeEphyon]

Nice vid, i'll do my own version :D

[Jon160]

In the video you implied that there is no net change of ATP.

There is actually a net gain of 2 ATP for every glucose, because there are 2x 1-3 BPGs. Therefore you get 4 ATPs by substrate level phosphorlation; a net gain of 2.

[harper277]

what about fructose 1,6 bisphosphate with aldolase dihydroxyacetone phosphate and the revrsable reaction with triose phosphate isomerase to glyceraldehyde 3 phosphate before moving on to two molecules of 1,3 bis phospho glycerate.

(Stryer, 2007)

[Arty0569]

You're right, he overlooked that.

[djenkins13]

Yes it is aldolase which cleaves the F-1,6-BP into 2x3C molecules

[jclod2]

i'm confused, i was pretty sure the aldolase splits it into to different molecules

[vanessaleb]

One question: What is the name of the product before the pyruvate? is it the phosphophenolpyruvate or phosphoenolpyruvate?Thank you

[slpng5]

Phosphoenolpyruvate

[ppd2000]

that was really good mate. My exam is tomorrow, i think i will pass now! haha

[kianatehrani]

You are an angel sent from the Biochemistry God(s). THANK YOU!

I have to memorize glycolysis for my biochem class and this helps TREMENDOUSLY!

[hadihadi9]

thanks for the video

[saleem085]

Thumbs up!

[jisaid08]

I havent put up any videos in about a month due to personal problems/ vacations but this week I will get back on the ball. Thanks for watching.

[saleem085]

Excitedly looking forward to having you 'back on the ball'.

[jisaid08]

I havent put up any videos in about a month due to personal problems/ vacations but this week I will get back on the ball. Thanks for watching.

[saleem085]

Thumbs up! Thanks for your efforts to share your knowledge. Please add more videos about all the major topics in Biochem.

[napcool]

Hey, First off good job with the video.....However there may be a need of some correction.

1- It is the aldolase reaction that cleaves the 6C to 2x 3C

2- You do not get G-3-P straigth away. Its

G-3-P and DHAP (which then becomes G-3-P)

3- The Net ATP is 2. You said 0 but then later on you said everything is double....It would be best mentioning that ATP doubles as well to give a net of 2.

Once again a good job. Looking forward to the mechanism.

[jisaid08]

I'll make some more videos soon, I was at the Red Sea slacking and thats why I haven't posted anyhing recently.

[rocknarock88]

Thank you for posting this video! It was great! Keep up the great work!

[gjwang]

Your video needs a correction. Adolase will convert Fructose 1,6-Biphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, which then with the use of a triose phosphate isomerase, the dihydroxyacetone phosphate will be converted into a second copy of glyceraldehyde 3-phosphate. Please clarify this part of the video as this part is important for understanding the structures and mechanism.

[ptpaget69]

I agree with Jisaid08, you should read a biochem book before posting stupid comments.

[iammrted]

It appears you are just copying from the book and not truly explaining the role of the enzymes. Just constructive criticism.

[jisaid08]

Im going to make further parts to this series where I draw out the molecules and explain mechanisms but Im limited to 10 minutes on youtube so I have to do it in parts.

[Treknologist]

I love biochem too! BIG THANKS! This is complicated stuff. However, it is one thing to have thems terms memorized, and another to truly understand how the transformation occurs.

Please note that this film is only the Embden-Meyerhof pathway, the most common means of glycolysis, in humans presumably.

[jisaid08]

Yeah I hope in a upcoming video to talk about the mechanisms in the pathway. My degree is actually in biochem. I hope to publish a series of biochem videos.

[Treknologist]

DO IT! I am hoping to program some molecular dynamics computer models that can illustrate such processes in "real" time.

[jisaid08]

Awesome I would love to see any programs you write. In college I was required to take a C++ course to graduate and I really enjoyed it. Sometimes in my spare time I try to make amature programs based on biochem. I emailed you my source code for my amino acid database. The code is probably extremely inefficient since its just a side hobby of mine but I none the less enjoy doing it.