DNA Test Methods - Hybridisation

[iLOVENATURE2011]

brief yet informative video about DNA testing and results. TY

[blazflare]

People use High Resolution Melt Analysis to test for DNA nowadays..

[bowelazop]

thank!!!! very good video

[Rigelcentauri58]

Very Good!!!, in only one minute you teach about this technic.

Your capacity for teaching is outstanding. Congratulations!!

[mehrshadpiano]

very helpful . nice job. now I got that .

[som3aelgen]

thanks a lot

[farahabdullah]

thank you :)

[baba7107]

@farahabdullah why the probe suddenly attaches to the strand of interest why not the complementary strand of the strand of interest anneal again

[SeoulSarah]

@baba7107 That's a very good question! Did you ever found out the answer?

[MaxWa]

@SeoulSarah & @baba7107

The chances are the strand of interest (hereafter SOI) will reanneal to both the original complementary strand and the probe. But if the probe is at a much higher concentration than the original DNA, more probe will anneal to SOI DNA rather than the complementary strand. Therefore, there will be a net fluorescence/radioactivity easy to isolate.

Another method is to use PCR with a primer specific to the SOI, giving a single predominant product.

Hope this helps :)

[CaminDaR]

@baba7107 because the complementary strand IS the strand of interest. The probe is a DNA strand of KNOWN sequence. So the probes that you choose to use in this technique will bind specifically with the segment of DNA that you are looking for.

[proteinP53]

good 5/5

[vanessaleb]

Thank you a lot.

[goneem]

thank you very much man

[AvengerArts]

thanks a lot