RNA Splicing

[jjclassjj]

interesting video and very informative

[rawwtheman]

i find this hard to masturbate to...

[PAE1966Gate13]

how is the exon actually caused to do the loop though?

[muawidan]

Thanks a lot for the video!!

[chernirm]

thank you for such an informative video!

[djjmria]

ok but how do those introns come back?

[Raikara]

@djjmria This isnt DNA, its mRNA, so the introns don't need to come back :). Oh and btw: they never come back. In some cells this happens also to DNA (for example in the immune system), then the DNA is irreversably changed. (--> thats why you cant clone from a white bloodcell)

[djjmria]

@Raikara thanks, too bad i failed my test with 1 wrong answer too much :( i thought mtDNA has overall less mutations than nuclear DNA over time..

[AnnaEvolut]

oh yea oh yea o yeah just what i was lookin for

[adares19]

It's a snRNP party!!!

[Genemaster13]

ATP is needed when U2 joins for the bending to occur. Further bending may occur when UA/5/6 joins.

The model in this movie is incorrect and not based on the experimental data that have been published (please check medline and primary data if you don't believe me)

[Genemaster13]

not accurate: RNA bent before U4/U5/U6 bind to RNA (RNA bent following U2 binding)

[DGrunst]

@Genemaster13 you are not accurate. U4/U5/U6 facilitate the bending of the RNA strand. The interaction between U2 and the U456 complex pulls U1 inward causing the bend.

[TheLethargic21]

Ahh! God send!

[gogoreddz]

im only 11 and i understood everything u said

[theazn07]

1:00 LULU LEMON

[purplesky001]

Not bad, but the rearrangement of the subunits to excise the intron was not as clear as it could be...

[DarkFacet]

what s the meaning of the "A" in the Intron, where the BBP and U2AF Protein are binding first? is it just some random adenin or is it a specific molecule?

[AZNinBLACK]

@DarkFacet it's the conserved adenine nucleotide in the intron mentioned at 1:01 ...it cleaves the sugar-phosphate backbone of RNA and is integral in the formation of the lariat structure.

[DarkFacet]

@AZNinBLACK thx a lot, but how can the protein find this conserved adenin, is it in a repeat sequence??

[hamaz]

@DarkFacet The conserved adenine sequence usually lies within a "consensus sequence" that is recognized by the BBP. Therefore the base-pairs around the adenine are important to recognition. This is similar to bacterial use of consensus sequences about promoter sequences in transcription. The consensus sequence is really important as some diseases are actually caused when creation of new splice sites which can erroneously splice the protein and leave some of the intron in or cleave some exon.

[DarkFacet]

@hamaz now i finally got it, thanks a lot!!

[hamaz]

@DarkFacet no problem and just as a correction i meant to say "erroneously splice the gene" instead of the protein. This would ultimately lead to either an incomplete or incorrectly folded protein.

[Mexisio22]

Snerp derp, derp derp that's all i hear

[Caytel]

Sneeerp

[carramoni]

It is excellent because i need details and here there are a lot!!!!

[elwoodwinn]

nice explanation

[AllphrncMetactzn]

very good and thanks

[gek232]

is this how we poop ?

[reeka411]

@gek232

I believe this is WHY we poop

:D

[jesca215]

@reeka411 lmao that's cute

[mimiandolini]

excellent

[zalmi1]

We just learned about this in class, and this video explains everything so clearly. If I could give it higher than 5 stars I would because 5 stars in an understatement.

[emilioj17]

Es el mejor video, los demas no estan del todo completos.

[wliu003]

nice of the best videos on youtube

[Uxoriouswidow]

Nice

[histologt]

excellnt!!!

[Nicksie22]

good video, finally one with some detail