Biuret's Test

[vinkodai]

it was not that bad:DD it helped me:DD thank youu @hksssciencebuzz

[katasandiable]

aduh kypa nih mendownloadx, mauk bnr, gaje lalu!

[Tl82T]

first thing i do off the sound

[hennaforeva]

whats the buiret test. i know its for proteins but what is it i know wht benedicts test is used for to identify if reducing sugar is present ?

[funnystuffenstufff]

@hennaforeva im not sure what you mean, but specifically biuret tests for the presence of peptide bonds. Peptide bonds link each glucose molecule in a protein.

[UKAkiba1]

@funnystuffenstufff peptide bonds link amino acids together?

[MultiPrincessD]

yeaaaaaaaaaa

[samsaras]

and, the fact that the video cam was zoomed onto the name of the reagent at 0:55 (sodium hydroxide) is very obvious that the hint was already given. what i mean is, that the student has wrongly switched up the order of reagents to be added to the sample. it should be sodium hydroxide first, followed by copper (II) sulphate. that's the simple answer to the question posed in the video.

[samsaras]

hong kah secondary school?????????

[MultiMido14]

2ml protein solution

2ml of naoh

shake and add 2 drops of copper sulphate 2%

[abcdrqs2]

It doesn't say what concentration of NaOH it is, but its probably fairly dilute, none the less, it probably won't be the end of you without gloves. And a 1% CuSO4 solution is essentially harmless.

By the way its the Biuret Test, not Biuret's Test. There was no scientist named Biuret. Biuret is a name for H2NC(O)NH2, essentially two (bi-) urea molecules bonded togethe

[tranthimydung203]

How can she be so careless when handling those chemicals without gloves

[syamkamarul]

hmmmm....she must use a lil amount of NaOH.....then she forgot to use CuSO4

[Alwkiller]

She also did not shake after each drop in the first one she used

[guitaro69]

she used the NaOH last! loser

[Cap1108]

@guitaro69 u don't have to add the word loser to ur answer. stop trolling.

[ThexxGodfather]

not an equal amount? maybe?