Added: 3 years ago
From: ndsuvirtualcell
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  • Wow these videos are awesome! So concise. Much better than my former biochemistry teacher! lol

  • Doesn't the bottom portion of ATPsynthase spin as well? My biochem book said that the 3 beta subunits are distinct in that one is always "empty" because its bound to the gamma subunit. Another is awaiting ADP + Pi to come along and be bound, and then the third holds the newly synthesized ATP. When the 3rd unit is turned enough that it has to bind to the gamma subunit, it releases ATP and binds gamma. then the beta unit gets another ADP, turn, makes atp, turn, release atp and bind gamma, etc etc

  • @JamesBarker85 The bottom portion of the ATP sythase doesn't spin, only the top part does. The top isn't symmetrical though, so it creates a mechanical change in conformation in the bottom, like a square peg twisting in a round opening, the 3 pairs of alpha/beta subunits are squished into different shapes. Each subunit pair are in a state different than the other two, one state is empty, one has ADP+Pi and the last one has ATP. the three pairs of subunits alternate states, depending their state.

  • I don't see any way this system could have possibly evolved.

  • then you should be watching videos on evolution

  • @ccbass22 Refer me to a good one.

  • Comment removed

  • Nice vid. Just watched this for my online bio class :)

  • I thought that each NADH produced 3 ATPs and each FADH2 produced 2 ATPs on average. If it requires three H+ to produce one ATP, then this would contradict the 38 ATPs produced on average from each glucose.

  • @rhoadess from viewing the ETC video, each NADH is responsible for pumping 6 hydrogen ions which would account for 2 ATPs, but according to this, it would require 9 hydrogen ions to make the needed 3 ATPs for each NADH. If we assume that 3 ATPs are made for each NADH, and we assume that one full rotation is required to make the 3 ATPs, then for each hydrogen ion, the rotor circumvents 60 degrees.

  • @rhoadess Not only NADH and FADH2 account for the ATP generation. Glucose goes through both glycolysis and kreb cycle.

  • MUY BUEN VIDEO

  • Very helpful animation! Thanks! Please create more (Kreb's cycle, Glycolysis, Glycogenolysis). :-)

  • Awesome vdo....!

  • Great!! Fantastic!!

  • hmm im still not getting it. Can someone explain to me whats happening when the hydrogen ions enter the atp synthase and how that produces and atp molecule?

  • @flyers4life123 as it spins, it activates catalytic sites at the stationary (bottom) knob. 3 of these catalytic sites join the phosphate to ATP. So every 3 spins, enough catalytic sites are activated in order to form ATP from phosphate and ADP.

  • I was taught that 4H are required to make 1 ATP. Is there a reason for the variation?

  • Great video!

    Thanks!!

  • evolution is a Lie

  • Fantastic!

    

  • good video. could do without the creepy opening though.

  • @BlackHockeyMonkey agreed aha

  • Hej ndsuvirtualcell - thank's for the video. It makes things pretty clear BUT I believe you made a small mistake.

    According to your video a flux of H+ is needed so that the ATPsynthase has enough energy to make ATP. It's said in "Biochemistry" from Stryer (2010) that the rotation is only needed to release the ATP. In other words it CAN be synthesised without a proton gradient but NOT released. (see "binding-change mechanism" from Paul Boyer)

    I might be wrong. Feedback, please.

  • @jim3xPRO I have a separate textbook that says the same thing (Lehninger 5e), I think you are correct.

  • @timmo1107 Score! The good old Stryer and Lehninger. First source when it comes to biochemistry!

  • @jim3xPRO yea ur probs rite but its only explained to an a-level standard i think so i guess its just simplyfying things down

  • with videos, these vague concepts are easier to understand...

    thank u for the efforts...I love it

  • why are there teachers in unions when there are these superior learning tools?

  • These vids are brilliant! Chemistry explained in a simple, and FAR more interesting way than any chemistry book I know of - gotta love it! ^^

  • 467 people were reading stryer 's biochemistry book lol :P

  • Your videos are so helpful! Thank you!

  • Really nice, thanks a lot!

  • so cells have little machines in their membranes?

  • Mindblowing

  • yeah please keep making videos so ican pass biology and never ever have to think about it ever again

  • @shannunnnnnnn Worse way to learn

    Not even learning really, lol

  • @KelThuzadJaden as a liberal arts major i HIGHLY doubt i'll ever need biology ever again.

  • @shannunnnnnnn What if some dude on the street came up to you and threatened to kill you w/ his gun unless you taught him how ATP synthases worked? What would you do?

    I thought so!

    xD

  • @fandanstan I'd ask him what he or she got for his eight birthday. Distracted? Yes. Win? Yes.

  • @shannunnnnnnn wow sums me up too

  • I LOVE YOU !!!!1

  • THANK YOU

  • great job really thank you

  • can you explane the three ways to create ATP.

  • I would like to thank this video 9999^999999 times , I couldnt not figure it out by reading the book.

  • Amazing animation

  • That answered my question about the C ring...thanx

  • so the H+ ions bind to the Fo unit or the F1 unit?

  • @TTliv87 to Fo

  • @TTliv87 Theu bind to the F0 unit which causes the F1 unit to rotatate , keeping in mind that fhat or every proton that has been added to the alpha subunit it cases a rotation in the beta subnit this is where an anticolcowise rotation occurs in which the beta subunit changes for 3 conformatios ( open , closed and loose)

  • Lol, 2 vids of roughly 4 minutes each explained to me what I couldn't understand in 2 hours of studying. Ty! :D

  • Really thank you, thank you. This was a 100% clear animation and summary of what I need to present tomorrow for a school project class. Currently working on the cell and got picked for the Mitochondrien. This surely helped a lot!

  • This video contains at least one big mistake. The energy of the proton gradient, transformed in mechanic energy isn't the reason for the assembly of ADP with Pi and ATP - this happens automatically by the catalytic envoirment which sinks the activations energy. Rather important is that the motion of the F0-Ring leads to a rotation of the gamma-unit, which has a "bulp" - this bulp rotates and pushes one ATP out of the Betasubunit.

  • @Snurbify1 Addition: Automatically might be the wrong description: spontanously should be more comfortable in reference to the free energy

  • @Snurbify1 Isn't the the rotation mechanical energy? I'm not saying you're wrong I just kind of don't understand the whole 'automatically/spontaneously' by catalytic environment which sinks the activations energy part. could you elaborate?

  • @kickingtenshi Maybe I'm late with my answer, but I hope you'll still read it! I refered to the fact that the speaker of the video claims that the energy of this process serves the atp condensation - Experiments have shown that also without any proton gradient there are a chemical balance of ATP and ADP in the binding sites of the ATP-Synthetase. The proton gradient supplies the "energy" needed for the rease of ATP from the binding site. This is called the "binding-site change mechanism"

  • @Snurbify1 therefore the role of the proton gradient is not to form ATP however to relase it from the tight conformation , the complex needs the energy from the protons to rotate the Fo complex allowing the relase of the ATP that had been formed ??

  • that was very helpful for photosynthesis light-dependent reactions, since ATP synthase is used there also. These are very good videos, and i agree with kashifk9 that you should continue to make them. it will help all the people in honors biology (or in my case 7th grade advanced biology).

  • You could work with sal of khan academy

  • Its great, thank you a lot ;). 

  • 178,647 people has crappy Biology teachers!!

  • well, this helped a little i guess.... im in honors biology 1, sooooooooo

  • doesn't 1 incoming H+ into the matrix produce 3 ATP per revolution of the ATP synthase?

  • @justynad

    2:03

    3 protons -> 1 ATP

  • @justynad 1 NADH gives 10H+ and 3 to 4 protons is needed to make 1 ATP

    You are thinking about 1 NADH is making 3 ATP

  • 1 incoming H+ lets 6 ATP's to be produced? how is that possible? isin't it just 3 ATPs produced per revolution after 1 H+ enters?

  • How do the protons know what to do?

  • Comment removed

  • @smoothpeople33

    the protons are forced by the chemical gradient (Gibbs free energy) to move into the mitochondrion. The only way (for the protons) into the mitochondrion is via the atp synthase.

  • @smoothpeople33 Random kinetic energy

  • @kobocha09 Biology is the only science that hasn't come to terms with the fact that randomness doesn't result in essentially perfect order

  • I wish I watched your vids while taking graduate biochem. I would have had a solid understanding then and probably wouldn't be trying to relearn everything for step 1

    Thanks a lot!

  • Heh, I love the little introductory music. Very dramatic.

  • I hated ths part n Bio chem b4 ... but nw Its easy pickns ....... thnx you tube !!!

  • very very very helpful in my studies, thank you !

  • All of your videos are very helpful! Thank you!

  • This...

    is..

    impressive

    Proteincomplex, something a human could never recreate

  • These videos are perfect for studying the ATP Synthesis! Thank you, thank you!

  • thank you!

  • I love Christina Johnson's narration!

  • this was so helpful!

  • Such a huge help. Thank you so much for doing these animations; they make the processes so much clearer than still pictures and pages of explanation or hours of lectures can.

  • erm is ATP Synthase the same as ATPase ?

  • @Mrryank27 Yes, someone asked that in my biology class today. I believe so.

  • By far the best descriptive videos of Chemical reactions! I have been studying biology for many years, and this is the only channel I can trust for reliable information, displayed in an interesting manner. Thank you!

  • WTF, it's magic :O

  • Some great animations. Be Cosmic.

  • excellent description. thanks.

  • Very nice. What tools did you use ?

  • Now I get it, thanks.

  • thank you so much for theses information

  • helped out alot.... you can actually see whats going on. Animation is great keep up the good work.. keep them coming, this actually makes biology more understandable, thank you

  • Really helpful- better than textbooks because you can actually see what's going on rather than just linking long, dry sentences to a small picture in the corner of a page. Thank you!

  • Brilliant!

  • this is brillaint

  • Comment removed

  • love u thanx alot...it helped a lot....it was awesum..i cud not get a wordfrm the books..thanx nyways

  • ATP synthesis

  • actually 4 H+ are needed for a 120 degree turn to produce 1 ATP

  • @wrestle85 You need 1 H+ to come into the matrix with HPO4- (co-transport) to give you Pi to join with ADP so though you only need 3 H+ to turn 120 degrees, your net equation requires 4 H+ per ATP synthesized.

  • excellent video!!!!

  • WOW i understand!!!

  • Many thanks for these animations! they have helped me, as a clinician, to better understand basic physiology for my exams and when trying to teach medical students! If only these were around when I was at medical school!

  • THANK YOUUUUUUUUUUUUUUUU

  • This video is way over simplified and down right incorrect in some aspects.

  • haha agreed, but decent for a fundamental learning.

  • @dw237606 Relax. Know the audience. If this was an all inclusive video of ATP Synthase it'd be hours long. You know alot of Biology but apparently not much practical knowledge.

  • We are the incredible machines, aren't we?!!!

  • this is awesome! it brings a two dimensional picture in the book into live. thank you!

  • the problem that i faced with the electron chain is that i cant imagine wats really goin on !

    am just reading n tryn to memorize the info

    but now ive had a clear image regardin this topic

    thnx alot :)

  • COOOL

  • this is a very well explained video. it good to have it explained rather than shown with music. thanks for upload this video. along with your other ones. =)

  • its no sad....its hypnotic!!! ur cells are like a factories jeje....

  • hey!!!this is incredible,im form argentina and this video help me to my next test...im studing now and tomorrow its the test jajaja...but thanks to this video i think that i will pass the test =P

  • I have been teaching respiration for decades, and this video is superb for the dynamic way that it shows the "chemo-osmoticly" driven synthesis of ATP. Thank you for the work that went into it, and for posting it for all to benefit from.

  • i like the dramatic music..

  • I have seen ALOT of videos and I really love your videos. Please continue to make them they really help a lot

  • AMAAaazzzingggg!

    helped a lot!

  • this really helps my master's thesis..thanks!

  • This helps so much with my AP Biology class!

  • i thought atp synthase was located on the thylakoid membrane in plant cells? it exists in mitochondrion as well?

  • Yes they do. The only difference is that plants start with oxidizing water (into oxygen) to create an electrochemical gradient, while animals start with oxidizing NADH. At least I'm almost sure. Need a second opinion.

  • Yes, it's an important part of oxidative phosphorylation- actually, it's the reason that the electron transport chain even exists. It pumps H+ ions out of the matrix, forming a gradient, and they come back to form ATP. Holy crap, I'm hella nerdy....

  • think about cellular respiration vs. photosynthesis

  • yes , animals also need energy

  • Comment removed

  • Actually photosynthesis and the electron txp chain are basically two similar (almost) system. And in any biological system the energy currency is ATP. Being that both utilizes flavoproteins and cytochromes (heme protein), so it is safe to conclude that both system has almost the same mechanism (atp synthase) for ATP production. The slight difference maybe one that photosynthesis requires photons to excite the photosystems (complex).

  • awesome. best ive seen

  • great work

  • Great Stuff!

  • very very helpful, thank you!

  • Thank you so much. Please keep making videos, they are very helpful!

  • awesome! Great explanation! This helped a lot, and it's so fascinating....

  • very cool how it rotates

  • finally! my god my book skips the steps

  • all thier vids, good stuff.

  • Very helpful, thanks.

  • great vid. really helpful and detailed and easy to understand!

  • thank you sooo much!

  • A great study aid! Thanks for posting this video.

  • "meravigliosa macchina molecolare"

  • This is ingenious!

  • i thought that it took 2 protons for every ATP produced, they say 3?

  • yes i learned 2 as well

  • thank god i found u, i was lost without u...

  • Jonathan Sarfati, PhD in Chemistry, and former Chess Champion of New Zealand, discusses how life could not have arisen without a "Designer" in his film "From Chemicals to living Cells". You can see a section of his teaching off our page under "Sarfati" - Chemicals to Cells. In the entire copy (not on our page) of his teaching, he also discusses how ATP could not occur without an Intelligent Designer.

  • Romans 1.....

    19 since what may be known about God is plain to them, because God has made it plain to them. 20 For since the creation of the world God's invisible qualities—his eternal power and divine nature—have been clearly seen, being understood from what has been made, so that men are without excuse.

  • omg thank youuu

  • Definately the best noob-friendly explanation of this process and the components involved. Awesome!

  • touche-best vidoe yet to show how this works-thanks for sharing!!!

  • EXCELLENT , Best yet! Great graphics, and easy to follow.

  • so great! thank you..whew!! i'm saved..finally makes some sense

  • Very clear and didatic video. Made me understand better how the ATP Synthase works. =D

  • The music in the beginning of these vids is creepy! The videos are the best though.

  • haha I know!! I thought I was the only person who thought this! I have to "fast forward" it, because it gives me shivers! and I agree fantastic videos!

  • this was so much better than my professor's slides. thanks!

  • i´m a mexican, and i do not understand english very well, but this movie let me be understood...thank you very much!!!

    it will help so much people than me!!! :)

    see yaa!!

  • it helps a lot!!!

    Thanks!!

  • ack! My brain hurts,I HATE homework DX but atleast it makes it easier 2 understabnd

  • wow that video makes it a lot easier to understand.

  • very useful, thx

  • confusing ass shit lol

  • 'Electron Transport Animation' by spmarshall69 makes more sense.

  • Something doesn't ad up. In this video they say that you need 3 Hydrogen ions to produce 1 ATP. It is known that NADH+ knocks 6 hydrogen ions outside the membrane producing 3 ATP when gradient is restored. According to this video it would only produce 2ATP. what is up with that?

  • (1) NADH+H from complex 1 pumps 4H to the P-side, Complex 3 pumps 4H to the P-side (via Qcycle), and Complex 4 pumps 2H. For a total of 10H on the P-side (intermembrane side) producing approx 3ATP. 1H rotates the F1 unit 120 degrees, requiring 360 degrees to make 1 ATP.

  • @dpcmonkey Hi, dpcmonkey, I cannot understand when and how 1 complex pumps 4H+ to the P-side, I'm reading Ville right now, but from all sources, including "Cellular Respiration (Electron Transport Chain)" animation I can see that 1 complex pumps 2 H+ [one H+ per one e-].Could you explain or show me reliable source for this information?Also,on animation which I mentioned above,complex 3 pumps 4 H+,but I believe second pair of electrons comes there from FADH from complex 2,not from NADH.

  • Thanks, my prof didn't explain rotor rotation properly.

  • you guys pwn. since my book blows, and so does my teacher, this is my way of studying for my AP bio final.

    thanks so much.

  • does this happend only in plants or....?

  • In animals too.

  • Thanks a lot now i understand this very well thank u

  • cheers

  • a really helpful and well crafted verbal and visual description! thanks.

  • what doe's ATP mean

  • Adenosine triphosphate - your body makes it for energy.

  • It's like gasoline, but for animals instead of cars, I guess.

  • nice vid

  • NDSU! I stayed there during Fargo nationals! Word.

    Great video.

  • I've spent hours today trying to understand this... and it didn't help much...

    thanks to your vids I got in in less than 10 minutes :D