The DNA is heated, denatured into two separate strands, 15 to 20 nucleotides are present in which they act as primers on the and 5' and 3' end, by doing this there is a middle section. Heat-resistant polymerase (from bacteria from the hot springs) does away with the rest of the dNA strand. The frequency at which a wanted sequence is easy to get by multiple replications. 3/25%, some issues are base pair mismatches or errors.
Had a lot of fun with the comments, ( I'm bilingual too ) - a very nice distraction from learning for biology und biologie =) Anyways, a very good video that helped me a bit more to understand the PCR, even if it was not very detailed. Thank you for that!
ok how do you make the primers, do you add A T G C in a sequenced mix in test tubes and heat treat to cause them to bind into the gene sequence that you want? how do we know that the right genes are being targeted, because at some point the A T G C repeat at different parts along the DNA strand, this process cannot directly find the genes, im guessing its multiple processes after the target strand is found so you take the smaller part and then subdived off of that to get precise gene pieces.
@zackboomer Sorry, I wrote it the German way! That can happen when your bilingual. So look in a German dictionary and you'll see it's spelt right.
Don't worry about little things like that. I'm glad though you knew what it meant. Ps. more corrections? If so so's no more time to answer. Ps. so's is short for ...... have a good guess!
@zackboomer Thanks Unkraut! You to are forgiven. Just inform yourself better next time before judging, or being Mr/Mrs brainy box! Mein Gott du hast nichts anderes zu tun als nachzuschauen wer was schreibt. Dein Leben muss mega langweilig sein.
@watashiwaka19501 i know that there are viral cutters and mending chemicals that chop up dna and push it together, because thats the same mechanisms that viruses use to hijack your cells, but from this process i think it has to have more than one PCR step to target more precise gene sequences. great video but i think some more important parts are not shown, yea there is got to be more to this, i wonder what the primers and polymerase steps are called.
No, its DNA strand which is being separated. RNA is usually single stranded. Remember simple rule? DNA is used to make RNA, which in turn, is used to make proteins.
DNALEARNINGCENTER IS SOOOOOOOOOOOOO HELPFUL. I LOVE IT!
p8chill 1 day ago
oh, so that's what they mean by amplified :) Only the desired portion is copied!
salunete 1 month ago
The DNA is heated, denatured into two separate strands, 15 to 20 nucleotides are present in which they act as primers on the and 5' and 3' end, by doing this there is a middle section. Heat-resistant polymerase (from bacteria from the hot springs) does away with the rest of the dNA strand. The frequency at which a wanted sequence is easy to get by multiple replications. 3/25%, some issues are base pair mismatches or errors.
FateStayNightOST 1 month ago
Not as informative as could be, but really beautiful
Basiriku 1 month ago in playlist Другие видео от пользователя DNALearningCenter
awesome video thank you! perfect for my Biology exam...
MilezJoBroluver94 3 months ago
what is the primer in the PCR?
isam1335 3 months ago
@isam1335 a synthetic one
Ebeet0FAN 3 months ago
Had a lot of fun with the comments, ( I'm bilingual too ) - a very nice distraction from learning for biology und biologie =) Anyways, a very good video that helped me a bit more to understand the PCR, even if it was not very detailed. Thank you for that!
Aleatha 5 months ago
I wonder how the temperature can go up and down so fast.
bitiminicik 6 months ago
3 people couldn't get the concept.
DkanDywoot 7 months ago
ok how do you make the primers, do you add A T G C in a sequenced mix in test tubes and heat treat to cause them to bind into the gene sequence that you want? how do we know that the right genes are being targeted, because at some point the A T G C repeat at different parts along the DNA strand, this process cannot directly find the genes, im guessing its multiple processes after the target strand is found so you take the smaller part and then subdived off of that to get precise gene pieces.
boxa888 1 year ago
This is the best biology video i have ever seen!!!you have more of those??
vitalodion 1 year ago
Thank you!!! This visualization helped a great deal for my Biologie homework. :-)
wallacemail 1 year ago
@wallacemail you can't even spell biology
zackboomer 11 months ago
@zackboomer Sorry, I wrote it the German way! That can happen when your bilingual. So look in a German dictionary and you'll see it's spelt right.
Don't worry about little things like that. I'm glad though you knew what it meant. Ps. more corrections? If so so's no more time to answer. Ps. so's is short for ...... have a good guess!
wallacemail 11 months ago
@wallacemail You are forgiven, Kraut.
zackboomer 11 months ago
@zackboomer Thanks Unkraut! You to are forgiven. Just inform yourself better next time before judging, or being Mr/Mrs brainy box! Mein Gott du hast nichts anderes zu tun als nachzuschauen wer was schreibt. Dein Leben muss mega langweilig sein.
OMG Biologie .....Biology ......ENTSPANNT DICH!!!
wallacemail 11 months ago
@wallacemail Sie sind ein Bündel und es nimmt 1 zweites, um auf eine youtube Anmerkung zu antworten. Dieses Argument ist unzulässig. Guter Tag.
zackboomer 11 months ago
@zackboomer ???? Never mind! Also Good Day
wallacemail 11 months ago
@zackboomer try to translate on your own not via google traslator... you kinda... wrote everything wrong!
MilezJoBroluver94 3 months ago
@MilezJoBroluver94 lol you think I care that I wrote some stupid language wrong?
zackboomer 3 months ago
@zackboomer you probably did because you replied. But keep thinking you're a cool dude because you don't care about stupid stuff like languages.
beamoflaser 3 months ago
@beamoflaser oh, the obvious "you care because you replied" response. I don't give a shit about german because it will never be useful for me.
zackboomer 3 months ago
@wallacemail @zackboomer Discussing on the internet is like winning the paraolympics, even if you win, you are still retarded.
ForYeensSake 9 months ago
@ForYeensSake Wow that's a new one. I probably have only heard that one about 4389747239374893 times since I started using the Internet.
vladimirhorowitz 4 months ago
@zackboomer that was the german word for Biology... Biologie
MilezJoBroluver94 3 months ago
THANK YOU!!!!
K3v1nPr0 1 year ago
THANK YOU. YOU JUST SAVED ME.
ashtreeh 1 year ago
Very good visualization! Gives a good insight of the process! :)
Bjulle2 1 year ago
where does the primers and polymerase came from?... were they added in the process?
watashiwaka19501 1 year ago
@watashiwaka19501
yeah, before you start the PCR reaction
snikkeltje 1 year ago
@watashiwaka19501 i know that there are viral cutters and mending chemicals that chop up dna and push it together, because thats the same mechanisms that viruses use to hijack your cells, but from this process i think it has to have more than one PCR step to target more precise gene sequences. great video but i think some more important parts are not shown, yea there is got to be more to this, i wonder what the primers and polymerase steps are called.
boxa888 1 year ago
Shouldn't it be short RNA strands, not DNA strands?
zanderez 1 year ago
Comment removed
navhindustani 1 year ago
@zanderez
No, its DNA strand which is being separated. RNA is usually single stranded. Remember simple rule? DNA is used to make RNA, which in turn, is used to make proteins.
Narkodrom 1 year ago