@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated.
Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )
@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.
@DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.
This goes much quicker with the MMI H&E Staining Kit Plus (very much).
molecularmachines09 5 days ago
5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents
txdude86 8 months ago
That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.
pauldamedtech 8 months ago
why that guy didnt differentiate or blue?
ASSHOLELA 8 months ago
Is this how babies are made
Azander137 11 months ago
I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )
TheRealHarsjan 11 months ago
@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated.
Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )
MrJazkiller 10 months ago 2
@MrJazkiller oo ok thanks very much indeed :-)
TheRealHarsjan 10 months ago
@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.
MrJazkiller 10 months ago 2
Very nice!
melonshower 1 year ago
I don't think you need to put the slide into the xylene so long when you are deparaffinizing it.
DerSchnurrbart 1 year ago
@DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.
Theonewhoclimbs28 1 year ago
@DerSchnurrbart yes i agree.
MrJazkiller 10 months ago
This was the coolest thing I have ever seen, you are awesome!!!!
barminki 1 year ago