Immunoflourescent Assay (indirect) IFA إميونوفلورسنت

[rahulsmart83]

i work on Igm monoclonal antibody againts breast cancer.... for positive control i used igm mab from ascitic fluids..for negative control i`d used media free serum... but the result was same with positive control..... both got green color...or is it i need to use a correct filter?

[rann82]

@rahulsmart83

it seems that u had a contamination

also you may have as you said a wrong filter , refering to the kit leaflit is solution :)

please inform me a soon as you can about your progress to share it with you :)

alla the best

[rann82]

@rahulsmart83 hummmm the +ve and -ve control must be provided with the kit ?

[rahulsmart83]

what you were used for negative control?

[rann82]

hi :)

actually that depend on the kit and manufacturer instruction

for me i use them freshly....

some student use an old Distillate water  in washing and they lose all the plate! i have no looong experience :)

[em8r]

Can you mix your solutions beforehandor do you have to make them fresh?  how long will they keep?