Early Tyrosine Phosphorylation and the Calcium and Protein Kinase C Mediated Signaling Pathways in T Lymphocytes

[VideoGameCoupons]

i hope i know these all...lol

[1006Will]

And thank you so much for this very informative video, please make more!!!

[vicobeltran]

Appreciate any advice (v.beltran@aims.gov.au)

cheers

VB

[vicobeltran]

For example in vitro after bioluminescence reaction, aequorin can be regenerated via EDTA and reducing agents to again bind calcium and produce photons, but I don’t know what happens in vivo, whether the cell have heaps of AEQ and deploys some of them every time the neural impulse release calcium or there is a mechanism of calcium detachment from the EF-hand proteins once they are no longer required

[vicobeltran]

I have a question: What happens with the calcium ions AFTER bound to the target protein: either a) they are sequestred by a chelating molecule and then returned to the ER or b) they remain bound to the target protein (ei Calmodulin, Aequorin)) and then the whole protein is recycled via ubiquitin pathway?

[1006Will]

@vicobeltran I think there will be some sort of a change which make the target proteins release the Ca ions after transcription factors are made and sent into the nucleus.

[ethanhines]

Amazing. Well done and brilliantly explained. Makes you think all of those different cellular factors coming together just so NFkB gets activated...