Basic idea behind gel electrophoresis

[MsEgypt1973]

This mechanism is very much similar to Chromatography which is a technique used for separation of mixtures in solution depending on the difference in polarity of the mixture components.

[withakay2]

Wow, I love you. This video literally made me pass my bio test.  THANK YOUU!

[thaovn36]

Thank you for this video

[Dmenjivar22]

thank you sir, very much appreciated

[dahmer1552000]

best explanation so far.

[TheWestsideThuglife]

respect bro.

[jdb768]

Clarity added to a murky subject. Thank you for the best explanation I've seen on this subject.

[MyObscureReality]

Amazing. Thank You.

[Brownin90s]

thank youuu!

[nicknatpadywack]

GOD BLESS YOU

[amethystamg]

Is this guy a robot? I mean, really, he sounds like one of those voice software things?

[ferkanal]

this is the best presentation, so HELPFUL

[JOHHILOR]

epic as presentation, thanks!

[metricwitch]

thanks so much!!! this was tremendously helpful!!! bravo!~

[evademan95]

THaaaaaAAaaaaaank you for such a clear explanation! ;'D i luvd it

[PinkFloyd457]

Thankyou!

[omololasho]

Great video. Very clear and understandable.

[laversj]

i loved you in the simpsons

[Natalie8098]

Perfect little revision video before my biomedical exams!! Thanks!

[D3ltaForce]

Excellent



[floxc1]

Wow, thank you so much! I understand this a whole lot more than when my biology teacher taught this...

[bertiemycat]

what i dont get is what the point is in knowing that they are different sizes?

[munkskunk100]

@bertiemycat: the only practical use is just comparing them in a forensic scene

[randomragerick]

ricky G was here! ;D

[sicbrick]

thanks a bunch

[CrazYVolumE]

very good vid!

[smileloverrr]

Thank you, this video is great!

[dmswll8543]

This is really helpful!! I didnt understand the wikipedia but I understood this video very well, thanx :)

[dmswll8543]

This is really helpful!! I didnt understand the wikipedia but I understood this video very well, thanx :)

[jfsisaacs]

great!

[lslowiczek1]

extremely helpful, thankyou!

[ipod9029]

does the concentration of the gel dat we are using affect the migration of the fragments ?

[TheFuturemd91]

@ipod9029, YES, usually a .7% is used to seperate smaller fragments whereas 1-2% are used for larger bands >500bp.

[TheFuturemd91]

@ipod9029, YES, usually a .7% is used to separate smaller fragments whereas 1-2% are used for larger bands >500bp.

[TheFuturemd91]

@ipod9029 YES, usually a .7% is used to separate smaller fragments whereas 1-2% are used for larger bands >500bp

[Heavensent1492]

that was really helpful

[laveolu]

Clarified science. If teaching were a damsel, you'll be the husband of miss world.

[afnypoo]

best explanation of electrophoresis on youtube thank you

[suparisattu]

this lecture is very clear and easy to understand

[Jasexxxxx]

"The biggest pieces are black"...

[Moonshield15]

@Jasexxxxx hurr

[mohammadpad]

Very nice! Thanks :)

[Adz795]

Best electrophoresis video!

[resonatingvoice]

This is very well created ... thanks ...

[suddendeviced]

Oh my I get it!!! Thx a lot 4 the vid, now I can clearly understand electrophoresis!!!

[canaan1967]

Where are the fragments or porteins from? Are they named beforehand?  Does each well represent a person's sample?

[TeMpleOfHaTe]

pretty awesome but i cant understand something... Lets say we do electrophoresis into 2 different dna. shouldnt they travell with the same speed as long as they different in mass and so the atractive of the pole?

[543cbb]

Thank you so much :) it was very clear and helpful!!!!

[shabbyteapot]

Excellent! really helps with my forensic science course work. :-) would love to print out some of the images!

[sekhmeta8]

Very well done! Thank you very much!

[27Fos]

Very well explained thank you! Would have liked an extra on the staining enzymes.

[LiangRome]

look up the words compose and comprise. strunk would not be happy.

but nice explanation. helped a lot

[BogMonkey53]

Lets say I extracted my own DNA from say my saliva and put it into a well. Since I have 46 slightly different chromosomes would that mean I would have 46 bands?

[misawu]

no, becuase each chromose is made up of a tremendous amount of DNA, which can be made up of several fragments, if we were to split them up. therefore you would see alot of lines.

[BogMonkey53]

@misawu really? I thought they consisted of just 2 strands wound up. So in electrophoresis they chop the DNA into segments? I have no idea how this is used in forensics since the difference in genes between one mans and another is so tiny.

[BogMonkey53]

Wouldn't the bigger fragments be the most negatively charged? If so how come they move the slowest?

[nemirn]

Inside the gel, there is a messy network of "gel fibers" which obstruct the DNA fragments... Smaller fragments have an easier time working through the fibers. The large fragments are hinders.

(Kind of like a filter or screen)

[BogMonkey53]

What are these DNA fragments? Are they whole chromosomes?

[yashukomu]

perfect explanation ... mann you shud have been my biology prof.

[AdictedToSleep]

Thank you very mush for the explanation, that was very helpful

[noselicious42]

Thank you very much; this made perfect sense! Besides, I love your voice pattern and personable diction!

[simkepet]

thanks for the explanation

[ouroumeu]

Very clear! Thank you.

[ali23t]

Thank you for the video. Now that we have seperated the DNA fragments (according to size) and determined their molecular weight, what is the next step? How is this information useful?

[pegsbored]

this helped me out soo much, I needed a good, basic understanding of this, and this video showed me that, thank you so much!

[c1zx]

Wow, biology I can understand! Thanks for the very clear lecture.