RESTRICT ENZYMES

[prenishap]

missing info about blunt ends

[gopuishere]

short nd nice

[timster112]

why is Vietnam written in full caps. like "we're in freaking VIETNAM!"

[Epenth]

they scan it like a scanner, son

[ariabm44]

how do the restriction enzymes read the appropriate sequence of nucleotides?i mean how do they know where the5' GAATTC3' sequence is?

[DollWreck]

This is love.

[atheliajames89]

ok bt it shld b mr precise

[H011ycR4p]

K honestly i fucking know this shit already i just need ot know the fucking tempering further on..

[H011ycR4p]

What the fuck? I thought this was supposed to be pornhub

[carlosbink]

uhm .... dude , .. is that it?

[GoYeo19]

what happened to the rest of the video?

[Gabriela93xD]

can anyone write what he is saying ? thnks =D

[naylornation]

man wtf? where is the rest of the vid?

dis is wack b!

[MatiasRuizG]

Excellent

[Eunus]

Excellent

[jaide84]

great video, this helps out alot, i have a test on tuesday on this! trying to get into nursing:)

[Nyingmu]

overhangs and blunt ends?

not triangles and weird shapes?

[awjan5]

what do you mean by triangles and weird shapes?

it's because the restriction enzyme cuts at a certain base pair on each strand of the dna helix, for example:

5'GAATT C3'

3'C TTAAG5'

this is an example of sticky ends, so it means that for instance, TTAAG is sticky becasue it can base pair to another chain.

blunt ends on the other hand are like:

5'GGG CCC3'

3'CCC GGG5'

so GGG or CCC on either side, can NOT bind to other dna sequeunces... hope that helps!

[Spacemonkeymojo]

LMAO, good point. This is very general, for the noobs lol.

[putsometoeon]

yeah.... short and to the point, thanks :)

[abhijit00004]

not bad. short and sweet.

[valkyrie607]

It should point out that the fragments of introduced DNA were also created using the same restriction enzyme.

[chumanho]

Not only sticky ends, blunt ends works too...

[polarbear60]

How do you know?

[skellni]

brief and informative

[Eunus]

exacly